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改进小RNA-seq：减少偏差，更好地检测2'-O-甲基RNA
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Genética
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JoVE Journal Genética
Improving Small RNA-seq: Less Bias and Better Detection of 2′-O-Methyl RNAs
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改进小RNA-seq：减少偏差，更好地检测2'-O-甲基RNA

DOI:
10.3791/60056-v

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08:49 min

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September 16, 2019

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, ,
1Institute for Integrative Biology of the Cell, UMR9198, CNRS CEA Univ Paris-Sud,Université Paris-Saclay

Capítulos

  • 00:04Título
  • 00:33Small RNA Isolation
  • 02:26Unligated 3’ Adapter Elimination
  • 04:41Gel Purification
  • 05:27Raw Sequence File Treatment
  • 06:06Trimmed Sequence Mapping
  • 06:58Results: Representative 2’-OMe RNA Detection
  • 08:18Conclusion

Summary

Tadução automática

Tadução automática

我们提出了详细的小RNA库修复协议，比标准方法的偏差小，对2'-O-甲基RNA的灵敏度提高。使用自制试剂可节省成本或使用试剂盒以方便使用本协议。

Tags

Small RNA-seqRNA Detection2′-O-Methyl RNAsUnbiased MethodsPlant MicroRNAsGel ElectrophoresisRNA ExtractionAdapter EliminationMagnetic Purification Beads

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