Journal
/
Imunologia e Infecção
/
Rensning af humane S100A12 og dets Ioninducerede oligomerer til Immuncelle stimulation
JoVE Journal
Imunologia e Infecção
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Imunologia e Infecção
Purification of Human S100A12 and Its Ion-induced Oligomers for Immune Cell Stimulation

Rensning af humane S100A12 og dets Ioninducerede oligomerer til Immuncelle stimulation

DOI:
10.3791/60065-v

•

12:55 min

•

September 29, 2019

•

, ,
1Department of Pediatric Rheumatology and Immunology,University Children’s Hospital

Capítulos

  • 00:05Título
  • 01:02Anion-exchange Chromatography
  • 03:53Calcium-dependent Hydrophobic-interaction Chromatography (HIC)
  • 05:26Removal of Endotoxin and Oligomer Separation
  • 07:20Functional Testing on Monocytes
  • 10:22Results: Oligomers Separation
  • 11:59Conclusion

Summary

Tadução automática

Tadução automática

Denne protokol beskriver en rensningsmetode for rekombinant tagfrit calcium bindende protein S100A12 og dets ioninducerede oligomerer for humane monocyt-stimulerings analyser.

Tags

Artificial IntelligenceAI Writing AssistantsContent GenerationCopywritingArticle WritingText GenerationAI-powered Writing

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/60065?article_type=v

Purification of Specific Cell Population by Fluorescence Activated Cell Sorting (FACS)
check_url/pt/60065?article_type=v

Quantitative Assessment of Immune Cells in the Injured Spinal Cord Tissue by Flow Cytometry: a Novel Use for a Cell Purification Method
check_url/pt/60065?article_type=v

Flow Cytometry Analysis of Immune Cells Within Murine Aortas
check_url/pt/60065?article_type=v

Isolering af Funktionel Cardiac immunceller
check_url/pt/60065?article_type=v

Human In Vitro Suppression as Screening Tool for the Recognition of an Early State of Immune Imbalance
check_url/pt/60065?article_type=v

Generering af lymfeknuder-fedt Pad kimærer for Studiet af lymfeknuder stromacelle Origin
check_url/pt/60065?article_type=v

Påvisning af fluorescerende Nanopartikel Interaktioner med Primary Immune cellesubpopulationer ved flowcytometri
check_url/pt/60065?article_type=v

En Semi-automatiske tilgang til Forberedelse Antibody Cocktails for immunfænotypisk Analyse af Human Perifer Blod
check_url/pt/60065?article_type=v

Isolering og flowcytometrisk analyse af immunceller fra iskæmisk Mouse Brain
check_url/pt/60065?article_type=v

Isolation, karakterisering og funktionelle Undersøgelse af Gingival Immune Cell Network

Read Article