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对TNBS介导肠纤维化发展的力学洞察与拉帕霉素抑制作用的评价
JoVE Journal
Imunologia e Infecção
This content is Free Access.
JoVE Journal Imunologia e Infecção
Mechanistic Insight into the Development of TNBS-Mediated Intestinal Fibrosis and Evaluating the Inhibitory Effects of Rapamycin
DOI:

10:21 min

September 12, 2019

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Capítulos

  • 00:04Título
  • 00:52Induction of TNBS Fibrosis and Rapamycin Treatment in Mice
  • 01:58Isolation of Intestinal Lamina Propria and Purification of Cx3Cr1+ Mononuclear Phagocytes
  • 04:01Purification of Mononuclear Phagocytes from the Collected Cells Purification: Magnetic Purification
  • 04:51Purification of Mononuclear Phagocytes from the Collected Cells Purification: FACS Sorting
  • 05:39Flow Cytometry Analysis
  • 07:39Results: Analysis of TNBS-Mediated Intestinal Fibrosis
  • 09:27Conclusion

Summary

Tadução automática

在这项研究中,我们描述了TNBS介导的肠道纤维化的详细过程,它具有与克罗恩纤维化类似的病理生理学。我们还讨论了这种方法,鉴于拉帕霉素促进抑制作用的肠道纤维化。

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