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Aperçu mécaniste du développement de la fibrose intestinale médiée par tNBS et évaluation des effets inhibiteurs de la rapamycine
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1Department of Molecular and Cellular Physiology,Albany Medical College, 2The IBD Center, Division of Gastroenterology, Department of Medicine,Albany Medical College, 3Department of Geriatrics, School of Medicine and Health Science,University of North Dakota, 4Department of Neuroscience and Experimental Therapeutics,Albany Medical College
Capítulos
- 00:04Título
- 00:52Induction of TNBS Fibrosis and Rapamycin Treatment in Mice
- 01:58Isolation of Intestinal Lamina Propria and Purification of Cx3Cr1+ Mononuclear Phagocytes
- 04:01Purification of Mononuclear Phagocytes from the Collected Cells Purification: Magnetic Purification
- 04:51Purification of Mononuclear Phagocytes from the Collected Cells Purification: FACS Sorting
- 05:39Flow Cytometry Analysis
- 07:39Results: Analysis of TNBS-Mediated Intestinal Fibrosis
- 09:27Conclusion
Dans cette étude, nous décrivons une procédure détaillée de la fibrose intestinale TNBS-négociée, qui montre la pathophysiologie comparable à Crohn' fibrose de s. Nous discutons également cette approche à la lumière de la rapamycine a facilité des effets inhibiteurs sur la fibrose intestinale.
