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Perspicacia mecanicista sobre el desarrollo de la fibrosis intestinal mediada por TNBS y la evaluación de los efectos inhibidores de la rapamicina
This content is Free Access.
1Department of Molecular and Cellular Physiology,Albany Medical College, 2The IBD Center, Division of Gastroenterology, Department of Medicine,Albany Medical College, 3Department of Geriatrics, School of Medicine and Health Science,University of North Dakota, 4Department of Neuroscience and Experimental Therapeutics,Albany Medical College
Capítulos
- 00:04Título
- 00:52Induction of TNBS Fibrosis and Rapamycin Treatment in Mice
- 01:58Isolation of Intestinal Lamina Propria and Purification of Cx3Cr1+ Mononuclear Phagocytes
- 04:01Purification of Mononuclear Phagocytes from the Collected Cells Purification: Magnetic Purification
- 04:51Purification of Mononuclear Phagocytes from the Collected Cells Purification: FACS Sorting
- 05:39Flow Cytometry Analysis
- 07:39Results: Analysis of TNBS-Mediated Intestinal Fibrosis
- 09:27Conclusion
En este estudio, describimos un procedimiento detallado de fibrosis intestinal mediada por TNBS, que exhibe fisiopatología comparable a la fibrosis de Crohn. También discutimos este enfoque a la luz de los efectos inhibidores facilitados por la rapamicina sobre la fibrosis intestinal.
