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慢性沙门氏菌感染诱发肠纤维化

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Chronic Salmonella Infection Induced Intestinal Fibrosis

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慢性沙门氏菌感染诱发肠纤维化

DOI:

10.3791/60068-v

•

08:40 min

•

September 22, 2019

•

Bernard C. Lo, Samuel B. Shin, Melina Messing, Kelly M. McNagny

¹The Biomedical Research Centre,University of British Columbia

Capítulos

00:04Título
01:30Preparation of Salmonella Typhimurium △AroA Cultures for Oral Gavage of Mice
03:16Assessment of Salmonella Burdens in Tissues
04:36Picrosirius Red Staining and Quantification of Collagen
05:43Results: Chronic Salmonella Infection Induced Intestinal Fibrosis
06:26Conclusion

Summary

Tadução automática

该协议描述了沙门氏菌驱动的肠道纤维化的小鼠模型，类似于克罗恩病的关键病理特征，包括跨体炎症和纤维化。该方法可用于评估使用维持在C57Bl/6遗传背景的突变小鼠改变纤维化结果的宿主因素。

Tags

Intestinal Fibrosis Chronic Inflammation Crohn’s Disease Salmonella Infection Mouse Model Oral Gavage Streptomycin LB Agar LB Broth Bacterial Culture Sterile Technique

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