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分离完整眼珠以获得整体眼表面组织，用于组织学检查和免疫组织化学
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Biologia
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JoVE Journal Biologia
Isolation of Intact Eyeball to Obtain Integral Ocular Surface Tissue for Histological Examination and Immunohistochemistry
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分离完整眼珠以获得整体眼表面组织，用于组织学检查和免疫组织化学

DOI:
10.3791/60086-v

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05:15 min

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October 20, 2019

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,
1State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center,Sun Yat-sen University

Capítulos

  • 00:04Título
  • 01:18Dissection of Eyeball with Intact Ocular Surface and Eyelids
  • 03:52Results: Histological Examination and Immunohistochemistry of Obtained Integral Ocular Surface Tissue
  • 04:48Conclusion

Summary

Tadução automática

Tadução automática

该协议描述了一种在相对完整位置分离眼睑、眼表面、前段和后段的小鼠眼球的方法。

Tags

Ocular SurfaceHistological ExaminationImmunohistochemistryMouseDissectionEyeballOrbital BonesEyelidsLensRetinaOcular Surface Epithelial SheetHistologyImmunostainingEuthanasiaOptic NerveBrainParaformaldehydePBSSkullOrbital BonesEthmoidFrontalMaxillary BonesForceps

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