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免疫球蛋白 G N-甘油分析通过超高性能液色谱分析

JoVE Journal
Medicina

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JoVE Journal Medicina

Immunoglobulin G N-Glycan Analysis by Ultra-Performance Liquid Chromatography

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免疫球蛋白 G N-甘油分析通过超高性能液色谱分析

DOI:

10.3791/60104-v

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11:01 min

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January 18, 2020

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Di Liu*¹, Xizhu Xu*², Yuejin Li, Jie Zhang, Xiaoyu Zhang, Qihuan Li, Haifeng Hou, Dong Li, Wei Wang^2,3, Youxin Wang

¹Beijing Key Laboratory of Clinical Epidemiology, School of Public Health,Capital Medical University, ²School of Public Health,Shandong First Medical University & Shandong Academy of Medical Sciences, ³School of Medical and Health Sciences,Edith Cowan University

Capítulos

00:05Título
01:42Immunoglobulin G (IgG) Isolation
03:57Glycan Release
05:25Glycan Labeling and Purification
07:30Hydrophilic Interaction Chromatography and Analysis of Glycans
08:52Results: UPLC Chromatogram and Structure
09:30Conclusion

Summary

Tadução automática

免疫球蛋白G（IgG）N-甘油采用亲水相互作用色谱UPLC的特征是。此外，IgG N-甘油的结构明显分离。这里介绍这个实验方法，以便它可以在研究环境中广泛使用。

Tags

Keywords: Immunoglobulin G N-glycan Analysis Ultra-performance Liquid Chromatography IgG Purification Protein G Monolithic Plate Sample Preparation Glycan Isomers Plasma Samples Glycan Analysis

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