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通过红外纳米光谱和原子力显微镜对单个蛋白质聚合进行特征化
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JoVE Journal Bioquímica
Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy
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通过红外纳米光谱和原子力显微镜对单个蛋白质聚合进行特征化

DOI:
10.3791/60108-v

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12:58 min

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September 12, 2019

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, , , ,
1Centre for Misfolding Diseases, Department of Chemistry,University of Cambridge, 2Institute of Biotechnology, Life Sciences Center,Vilnius University, 3Cavendish Laboratory, Department of Physics,University of Cambridge

Capítulos

  • 00:04Título
  • 01:09Atomic Force Microscopy (AFM) Protein Aggregate Morphology Imaging
  • 05:09Infrared (IR) Nanospectroscopy Protein Aggregate Measurement
  • 11:57Results: Individual Protein Aggregated Characterization by IR Nanospectroscopy and AFM
  • 13:30Conclusion

Summary

Tadução automática

Tadução automática

我们描述了红外纳米光谱学和高分辨率原子力显微镜的应用，将蛋白质自组装过程可视化为寡聚体和淀粉样纤维蛋白，这与发病和发展密切相关广泛的人类神经退行性疾病。

Tags

Protein AggregatesInfrared NanospectroscopyAtomic Force MicroscopySingle Molecule MethodsBiomolecular ProcessesNano ScaleHeterogeneous SystemsProtein AggregationBiomaterialsDrug DeliveryAFM ParametersAFM ImagingAFM CantileverAFM Laser BeamAFM Deflected BeamAFM OscillationAFM Resonance

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