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JoVE Journal Developmental Biology
Microinjection of DNA into Eyebuds in Xenopus laevis Embryos and Imaging of GFP Expressing Optic Axonal Arbors in Intact, Living Xenopus Tadpoles
 

Microinjection of DNA into Eyebuds in Xenopus laevis Embryos and Imaging of GFP Expressing Optic Axonal Arbors in Intact, Living Xenopus Tadpoles

Article DOI: 10.3791/60123-v • 06:32 min • September 4th, 2019
September 4th, 2019 •
1, 1, 1
1College of Osteopathic Medicine, Touro University California

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This protocol aims to demonstrate how to microinject a DNA/DOTAP mixture into eyebuds of one day old Xenopus laevis embryos, and how to image and reconstruct individual green fluorescent protein (GFP) expressing optic axonal arbors in tectal midbrains of intact, living Xenopus tadpoles.

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Keywords: Microinjection DNA Xenopus Laevis Embryos GFP Optic Axonal Arbors Tadpoles Transgenesis Cell-specific Vertebrate Model Glass Micropipette Mineral Oil DOTAP De-vitellinization Embryo Transfer
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