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单细胞微吸气作为荧光激活细胞分类的替代策略，用于巨型病毒混合物分离
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Biologia
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JoVE Journal Biologia
Single Cell Micro-aspiration as an Alternative Strategy to Fluorescence-activated Cell Sorting for Giant Virus Mixture Separation
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单细胞微吸气作为荧光激活细胞分类的替代策略，用于巨型病毒混合物分离

DOI:
10.3791/60148-v

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09:50 min

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October 27, 2019

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, , , , ,
1Institut Hospitalo-Universitaire (IHU) – Méditerranée Infection, 2Microbes, Evolution, Phylogeny and Infection (MEΦI), Aix-Marseille Université UM63, Institut de Recherche pour le Développement IRD 198,Assistance Publique – Hôpitaux de Marseille (AP-HM)

Capítulos

  • 00:04Título
  • 01:37Amoeba Culture
  • 02:44Propagation of Stock Virus in Amoebae
  • 03:33End-point Dilution and Single Cell Micro-aspiration
  • 06:09PCR Screening
  • 07:06Results: Optimization of the Isolation of Novel Viruses
  • 08:17Conclusion

Summary

Tadução automática

Tadução automática

在这里，我们描述了一个单细胞微吸入方法分离受感染的阿米巴。为了分离受福斯托病毒和未知巨病毒感染的Vermamoeba病毒的病毒亚群，我们开发了下面详述的规程，并展示了其分离两种低丰度的新型巨病毒的能力。

Tags

Single Cell Micro-aspirationFluorescence-activated Cell SortingGiant VirusVirus SeparationVirus IsolationTurnover VirusClandisinal VirusUzibati VirusFaustovirusIndirect StrategyInfected HostBiologia MolecularElectronic MicroscopyVermamoeba VermiformisPYG MediumStarvation MediumMultiplicity Of Infection

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