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Enkelt celle mikro-aspiration som en alternativ strategi for fluorescens-aktiverede celle sortering for Giant virus blanding adskillelse
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Biologia
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JoVE Journal Biologia
Single Cell Micro-aspiration as an Alternative Strategy to Fluorescence-activated Cell Sorting for Giant Virus Mixture Separation

Enkelt celle mikro-aspiration som en alternativ strategi for fluorescens-aktiverede celle sortering for Giant virus blanding adskillelse

DOI:
10.3791/60148-v

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09:50 min

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October 27, 2019

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, , , , ,
1Institut Hospitalo-Universitaire (IHU) – Méditerranée Infection, 2Microbes, Evolution, Phylogeny and Infection (MEΦI), Aix-Marseille Université UM63, Institut de Recherche pour le Développement IRD 198,Assistance Publique – Hôpitaux de Marseille (AP-HM)

Capítulos

  • 00:04Título
  • 01:37Amoeba Culture
  • 02:44Propagation of Stock Virus in Amoebae
  • 03:33End-point Dilution and Single Cell Micro-aspiration
  • 06:09PCR Screening
  • 07:06Results: Optimization of the Isolation of Novel Viruses
  • 08:17Conclusion

Summary

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Her beskriver vi en enkelt celle mikro-aspiration metode til adskillelse af inficerede amoebae. For at adskille virale subpopulationer i Vermamoeba vermiformis inficeret af Faustovira og ukendte gigantiske vira, vi udviklede protokollen nedenfor og viste sin evne til at adskille to lav-overflod roman Giant vira.

Tags

Single Cell Micro-aspirationFluorescence-activated Cell SortingGiant VirusVirus SeparationVirus IsolationTurnover VirusClandisinal VirusUzibati VirusFaustovirusIndirect StrategyInfected HostBiologia MolecularElectronic MicroscopyVermamoeba VermiformisPYG MediumStarvation MediumMultiplicity Of Infection

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