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Isolering af leukocytter fra modermælken til brug i en antistofafhængig cellulær fagocytose analyse af HIV-mål
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Isolation of Leukocytes from Human Breast Milk for Use in an Antibody-dependent Cellular Phagocytosis Assay of HIV Targets

Isolering af leukocytter fra modermælken til brug i en antistofafhængig cellulær fagocytose analyse af HIV-mål

DOI:
10.3791/60149-v

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08:12 min

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September 06, 2019

•

,
1Division of Infectious Diseases,Icahn School of Medicine at Mount Sinai

Capítulos

  • 00:04Título
  • 00:44Target Microsphere Preparation
  • 02:20Antibody-Dependent Cellular Phagocytosis (ADCP) Assay Plate Preparation
  • 02:53Breast Milk Cell Isolation
  • 03:48ADCP Assay
  • 05:11Flow Cytometric Analysis
  • 06:21Results: Representative Flow Cytometric and ADCP Analyses of HIV Targets
  • 07:42Conclusion

Summary

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Modermælk overfører humant immundefektvirus (HIV), men kun ~ 15% af spædbørn ammet af HIV-inficerede mødre bliver smittet. Ammede spædbørn indtager ~ 105− 108 maternel leukocytter dagligt, selv om disse celler er undersøgt. Her beskriver vi isolationen af brystmælk leukocytter og en analyse af deres fagocytiske kapacitet.

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