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A Fluorescence-based Assay for Characterization and Quantification of Lipid Droplet Formation in Human Intestinal Organoids
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Medicina
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JoVE Journal Medicina
A Fluorescence-based Assay for Characterization and Quantification of Lipid Droplet Formation in Human Intestinal Organoids

A Fluorescence-based Assay for Characterization and Quantification of Lipid Droplet Formation in Human Intestinal Organoids

DOI:
10.3791/60150-v

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10:12 min

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October 13, 2019

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, ,
1Division of Pediatrics, Department of Pediatric Gastroenterology, Wilhelmina Children’s Hospital,University Medical Center Utrecht, Utrecht University, 2Regenerative Medicine Center,University Medical Center Utrecht, Utrecht University, 3Science for Life Laboratory, Department of Medical Biochemistry and Microbiology,Uppsala University

Capítulos

  • 00:04Título
  • 01:15Culture Procedures for Human Small Intestinal Organoids
  • 04:05Lipid Droplet Formation Assay
  • 07:26Results: Analysis of Lipid Drop Formation
  • 09:29Conclusion

Summary

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This protocol describes an assay for the characterization of lipid droplet (LD) formation in human intestinal organoids upon stimulation with fatty acids. We discuss how this assay is used for quantification of LD formation, and how it can be used for high throughput screening for drugs that affect LD formation.

Tags

Keywords: Lipid DropletIntestinal OrganoidsFluorescence AssayDGAT1 DeficiencyHigh-throughput ScreeningLipid MetabolismCell CultureTrypsinizationCell Counting

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