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人类肠道器官脂滴形成特征化和定量的荧光测定

JoVE Journal
Medicina

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JoVE Journal Medicina

A Fluorescence-based Assay for Characterization and Quantification of Lipid Droplet Formation in Human Intestinal Organoids

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人类肠道器官脂滴形成特征化和定量的荧光测定

DOI:

10.3791/60150-v

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10:12 min

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October 13, 2019

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Jorik M. van Rijn^2,3, Marliek van Hoesel², Sabine Middendorp²

¹Division of Pediatrics, Department of Pediatric Gastroenterology, Wilhelmina Children’s Hospital,University Medical Center Utrecht, Utrecht University, ²Regenerative Medicine Center,University Medical Center Utrecht, Utrecht University, ³Science for Life Laboratory, Department of Medical Biochemistry and Microbiology,Uppsala University

Capítulos

00:04Título
01:15Culture Procedures for Human Small Intestinal Organoids
04:05Lipid Droplet Formation Assay
07:26Results: Analysis of Lipid Drop Formation
09:29Conclusion

Summary

Tadução automática

该协议描述了在脂肪酸刺激下人体肠道器官中脂液滴（LD）形成的表征测定。我们讨论了这种测定如何用于LD形成定量，以及如何用于影响LD形成的药物的高通量筛选。

Tags

Keywords: Lipid Droplet Intestinal Organoids Fluorescence Assay DGAT1 Deficiency High-throughput Screening Lipid Metabolism Cell Culture Trypsinization Cell Counting

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