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严格厌氧/缺氧条件下微生物培养的介质制备
JoVE Journal
Bioquímica
This content is Free Access.
JoVE Journal Bioquímica
Medium Preparation for the Cultivation of Microorganisms under Strictly Anaerobic/Anoxic Conditions
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严格厌氧/缺氧条件下微生物培养的介质制备

DOI:
10.3791/60155-v

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06:17 min

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August 15, 2019

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, , , , , ,
1Department of Microbiology,Universität Innsbruck

Capítulos

  • 00:04Título
  • 00:39Preparation of Medium and Filling of Cultivation Flasks
  • 02:29Inoculation of the Medium
  • 03:38Incubation, Sampling, and Analysis
  • 04:57Results: Microorganism Cultivation under Anaerobic Conditions
  • 05:48Conclusion

Summary

Tadução automática

Tadução automática

由于义务厌氧生物在氧气暴露后无法生长,使用厌氧培养技术是必不可少的。在这里,我们演示了一种简单而有效的方法,从沼气厂到气体和挥发性脂肪酸定量,培养从沼气厂衍生的混合培养物。

Tags

AnaerobicAnoxicOxygen-freeREDOX IndicatorReducing AgentAnaerobic DigesterSludgeSerum FlaskInoculumLiquid CultureMedium PreparationMicroorganism Cultivation

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