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Generation of Chimeric Axolotls with Mutant Haploid Limbs Through Embryonic Grafting
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Genética
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JoVE Journal Genética
Generation of Chimeric Axolotls with Mutant Haploid Limbs Through Embryonic Grafting

Generation of Chimeric Axolotls with Mutant Haploid Limbs Through Embryonic Grafting

DOI:
10.3791/60156-v

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07:17 min

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January 29, 2020

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, ,
1Department of Molecular, Cellular, and Developmental Biology,Yale University

Capítulos

  • 00:05Título
  • 00:48Female Gamete Donor Preparation
  • 01:36Male Gamete Collection and Preparation
  • 02:51Female Gamete Collection
  • 03:48Haploid-Diploid Chimera Generation
  • 05:07Results: Representative Haploid and Diploid Limb Development
  • 06:30Conclusion

Summary

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This goal of this protocol is to produce chimeric axolotls with haploid forelimbs derived from Cas9-mutagenized donor tissue using embryonic tissue grafting techniques.

Tags

Chimeric AxolotlsHaploid LimbsEmbryonic GraftingGenetic ScreeningRegenerationAmphibianIn Vitro FertilizationGamete DonorChorionic GonadotropinSpermic UrineSperm IrradiationCrosslinker

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