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通过胚胎移植，生成具有突变倍体肢体的奇美体轴球

JoVE Journal
Genética

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JoVE Journal Genética

Generation of Chimeric Axolotls with Mutant Haploid Limbs Through Embryonic Grafting

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通过胚胎移植，生成具有突变倍体肢体的奇美体轴球

DOI:

10.3791/60156-v

•

07:17 min

•

January 29, 2020

•

Lucas D. Sanor, G. Parker Flowers, Craig M. Crews

¹Department of Molecular, Cellular, and Developmental Biology,Yale University

Capítulos

00:05Título
00:48Female Gamete Donor Preparation
01:36Male Gamete Collection and Preparation
02:51Female Gamete Collection
03:48Haploid-Diploid Chimera Generation
05:07Results: Representative Haploid and Diploid Limb Development
06:30Conclusion

Summary

Tadução automática

该协议的目标是使用胚胎组织移植技术，从Cas9诱变的供体组织中提取单倍体前肢的嵌合体轴片。

Tags

Chimeric Axolotls Haploid Limbs Embryonic Grafting Genetic Screening Regeneration Amphibian In Vitro Fertilization Gamete Donor Chorionic Gonadotropin Spermic Urine Sperm Irradiation Crosslinker

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