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Isolement de vésicules extracellulaires enrichies en exosomes transportant un facteur de stimulation des colonies de granulocytes-macrophages à partir de cellules souches embryonnaires
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1Department of Pharmacology and Toxicology,University of Louisville, 2Experimental Therapeutics Program, Brown Cancer Center,University of Louisville, 3Department of Medicine,University of Louisville, 4Department of Surgery,University of Louisville, 5Immuno-Oncology Program, Brown Cancer Center,University of Louisville, 6Department of Microbiology and Immunology,University of Louisville
Capítulos
- 00:04Introduction
- 01:01ES-D3 Cell Culture
- 03:06Exosome-Enriched Extracellular Vesicle (EV) Isolation
- 04:09Exosome-Enriched EV Transmission Electron Microscopy (TEM) Characterization
- 05:12Whole Cell Extract (WCE) and EV Lysate Preparation
- 06:29Western Blot Analysis
- 07:11Enzyme-Linked Immunosorbent Assay (ELISA)
- 08:48Results: Representative GM-CSF Expression Analyses
- 11:13Conclusion
