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Infecting Mice with Malassezia spp. to Study the Fungus-Host Interaction
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Infecting Mice with Malassezia spp. to Study the Fungus-Host Interaction
DOI:

06:19 min

November 06, 2019

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Capítulos

  • 00:04Título
  • 01:02Malassezia Inoculum Preparation
  • 02:26Malassezia Infection
  • 03:29Malassezia-Induced Skin Inflammation and Fungal Burden Analysis
  • 04:35Results: Representative Malassezia Skin Infection Analyses
  • 05:23Conclusion

Summary

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This protocol outlines the establishment of a mouse model for studying Malassezia-host interactions in the skin. It describes the cultivation of Malassezia in vitro, the infection of the murine skin with Malassezia, and the subsequent analysis of the inflammation and the fungal burden in the skin tissue.

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