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Targeting Drugs aan Larvale Zebrafish Macrofagen door injecterendrug-loaded Liposomen
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Targeting Drugs to Larval Zebrafish Macrophages by Injecting Drug-Loaded Liposomes
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Targeting Drugs aan Larvale Zebrafish Macrofagen door injecterendrug-loaded Liposomen

DOI:
10.3791/60198-v

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11:31 min

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February 18, 2020

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, , , , ,
1Department of Molecular Medicine and Pathology, Faculty of Medical and Health Sciences,University of Auckland, 2School of Pharmacy, Faculty of Medical and Health Sciences,University of Auckland, 3School of Medicine, Faculty of Medical and Health Sciences,University of Auckland

Capítulos

  • 00:05Introduction
  • 01:20Drug-loaded Marina Blue-Labeled Lipsome Preparation
  • 03:24Liposome Size and Zeta Potential Characterization and Entrapment Efficiency and Drug Loading Calculation
  • 04:11Preparation and Liposome Injection
  • 06:47Confocal Imaging and Image Analysis
  • 09:03Results: Representative Effects of Drug-loaded Lipsome Injection
  • 10:48Conclusion

Summary

Tadução automática

Tadução automática

Hier beschrijven we de synthese van door drugs geladen liposomen en hun micro-injectie in larve zebravissen met als doel de levering van geneesmiddelen aan macrofaagcellen te richten.

Tags

LiposomesMacrophagesZebrafishDrug DeliveryMitochondrial Reactive Oxygen SpeciesPoloxamer 188ExtrusionUltracentrifugationLive Cell Imaging

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