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Biologia do Desenvolvimento

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杏中自体和（内）相容关系的确定结合手授、显微镜和遗传分析

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Biologia do Desenvolvimento

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JoVE Journal Biologia do Desenvolvimento

Determination of Self- and Inter-(in)compatibility Relationships in Apricot Combining Hand-Pollination, Microscopy and Genetic Analyses

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杏中自体和（内）相容关系的确定结合手授、显微镜和遗传分析

DOI:

10.3791/60241-v

•

08:08 min

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June 16, 2020

•

Sara Herrera², Jorge Lora, José I. Hormaza, Javier Rodrigo²

¹Unidad de Hortofruticultura,Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), ²Instituto Agroalimentario de Aragón – IA2 (CITA-Universidad de Zaragoza), ³Instituto de Hortofruticultura Subtropical y Mediterránea La Mayora (IHSM La Mayora-UMA-CSIC)

Capítulos

00:04Introduction
00:52Self- and Cross-Pollination in the Laboratory
02:14Evaluating Pollen Viability through In Vitro Pollen Germination
02:30Evaluating Self(in)compatibility by the Observation of Pollen Tube Growth
03:22S-allele Identification
05:40Results: Determination of Self-(in)compatibility and Identification of S-genotype in Apricot Cultivars
06:58Conclusion

Summary

Tadução automática

本文提出了一种方法，将荧光显微镜的自（in）相容性与通过PCR分析识别S-基因型相结合的方法，确定杏（Prunus亚美尼亚卡L.）的授粉要求。

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