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Imunologia e Infecção
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Implantation and Monitoring by PET/CT of an Orthotopic Model of Human Pleural Mesothelioma in Athymic Mice
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Implantation and Monitoring by PET/CT of an Orthotopic Model of Human Pleural Mesothelioma in Athymic Mice

Implantation and Monitoring by PET/CT of an Orthotopic Model of Human Pleural Mesothelioma in Athymic Mice

DOI:
10.3791/60272-v

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07:54 min

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December 21, 2019

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, , , ,
1MicroPET/SPECT/CT Imaging Laboratory, Centre for BioMedical Imaging (CIBM), Division of Nuclear Medicine and Molecular Imaging,University Hospitals and University of Geneva, 2Division of Thoracic and Endocrine Surgery,University Hospitals and University of Geneva

Capítulos

  • 00:05Título
  • 00:45Tumor Cell Implantation
  • 02:43[18F]FDG-PET/CT Imaging
  • 04:12Analysis of [18F]FDG-PET/CT Scans
  • 06:08Results: Malignant Pleural Mesothelioma Monitoring with [18F]FDG-PET/CT Imaging
  • 06:59Conclusion

Summary

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This article describes the generation of an orthotopic mouse model of human pleural mesothelioma by implantation of H2052/484 mesothelioma cells into the pleural cavity of immunocompromised athymic mice. The longitudinal monitoring of the development of intrapleural tumors was assessed by non-invasive multimodal [18F]-2-fluoro-2-deoxy-D-glucose positron emission tomography and computed tomography imaging.

Tags

Keywords: Orthotopic ModelPleural MesotheliomaAthymic MicePET/CTImplantationMonitoringAnesthesiaBuprenorphinePovidone-iodineCell SuspensionHamilton SyringeAbsorbable SuturesFDGDose CalculatorRadioactivity

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