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通过高分辨率共聚焦显微镜对3D肿瘤球体入侵的影响表征
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Pesquisa do Câncer
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JoVE Journal Pesquisa do Câncer
Characterization of the Effects of Migrastatic Inhibitors on 3D Tumor Spheroid Invasion by High-resolution Confocal Microscopy
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通过高分辨率共聚焦显微镜对3D肿瘤球体入侵的影响表征

DOI:
10.3791/60273-v

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09:17 min

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September 16, 2019

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, ,
1School of Applied Sciences,University of Huddersfield, 2Laboratory of Radiotherapy and Experimental Radiation Oncology, Humbertus Wald Tumorzentrum,University Cancer Center Hamburg, University Medical Center Hamburg-Eppendorf

Capítulos

  • 00:04Título
  • 01:01Generation of Cell Spheroids
  • 02:15Collagen Invasion Assay
  • 04:15Preparation of Collagen Embedded Spheroids and Migratory Cells for Confocal Microscopy
  • 07:13Results: Cell Migration and Invasion
  • 08:20Conclusion

Summary

Tadução automática

Tadução automática

使用组蛋白脱乙酰雅酶（HDAC）抑制剂的三维（3D）入侵测定中，压伤抑制剂对胶质瘤癌细胞迁移的影响以高分辨率共聚焦显微镜为特征。

Tags

3D Tumor SpheroidMigrastatic InhibitorsConfocal MicroscopyPesquisa do CâncerCytotoxic DrugsCell MigrationCell Proliferation3D Cell CultureGliomaCollagenCell Invasion

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