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普帕尔阶段发育性皮瘤中组织定向和生长动力学的成像与分析

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Biologia do Desenvolvimento

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JoVE Journal Biologia do Desenvolvimento

Imaging and Analysis of Tissue Orientation and Growth Dynamics in the Developing Drosophila Epithelia During Pupal Stages

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普帕尔阶段发育性皮瘤中组织定向和生长动力学的成像与分析

DOI:

10.3791/60282-v

•

08:25 min

•

June 02, 2020

•

Federica Mangione², Enrique Martin-Blanco

¹Instituto de Biología Molecular de Barcelona, Consejo Superior de Investigaciones Científicas,Parc Científic de Barcelona, ²The Francis Crick Institute

Capítulos

00:05Título
01:06Live Imaging of Growing Abdominal Epithelia
03:24Generation of Genetic Mosaics to Follow Behaviors of Cell Clones
04:23Data Processing and Analyses
05:57Results: Long-term Live Imaging and Clonal Analysis
07:22Conclusion

Summary

Tadução automática

该协议旨在成像和分析果蝇在果蝇发生变质时，果蝇腹部上皮细胞定向和组织生长动力学。本文描述的方法可用于研究果蝇或其他模型生物体的不同发育阶段、组织和亚细胞结构。

Tags

Growth Dynamics Drosophila Epithelia Pupal Stages Cell Behavior Morphogenesis Planar Polarity Live Imaging Drosophila Pupal Epidermis

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