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在五个脊椎动物群中可靠隔离中枢神经系统微血管

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Reliable Isolation of Central Nervous System Microvessels Across Five Vertebrate Groups

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在五个脊椎动物群中可靠隔离中枢神经系统微血管

DOI:

10.3791/60291-v

•

10:35 min

•

January 12, 2020

•

Yinyu Yuan*¹, Jacquelyn R. Dayton*¹, Marie-Lena Freese, Bryce G. Dorflinger, Lillian Cruz-Orengo

¹Department of Anatomy, Physiology and Cell Biology,University of California Davis, ²University of Veterinary Medicine Hannover Foundation

Capítulos

00:04Título
00:40Central Nervous System (CNS) Tissue Dissection
02:35CNS Tissue Homogenization
04:20Microvessel Purification
05:57Microvessel Elution and Filtration
07:44Results: Representative Microvessel Protein Expression
09:57Conclusion

Summary

Tadução automática

该协议的目的是从脑血管和陀螺脊椎动物的中枢神经系统的多个区域分离微血管。

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Keywords: Microvessel Isolation Central Nervous System Vertebrates Dissection Meninges Choroid Plexus Homogenization

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