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Migration, Chemo-Attraction, and Co-Culture Assays for Human Stem Cell-Derived Endothelial Cells and GABAergic Neurons
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Neurociência
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JoVE Journal Neurociência
Migration, Chemo-Attraction, and Co-Culture Assays for Human Stem Cell-Derived Endothelial Cells and GABAergic Neurons

Migration, Chemo-Attraction, and Co-Culture Assays for Human Stem Cell-Derived Endothelial Cells and GABAergic Neurons

DOI:
10.3791/60295-v

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10:09 min

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January 23, 2020

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,
1Department of Psychiatry,Harvard Medical School, 2Angiogenesis and Brain Development Laboratory, Division of Basic Neuroscience,McLean Hospital

Capítulos

  • 00:05Título
  • 01:17Preparation of Human Cells for Assay
  • 03:36Preparation of One-Well Culture Inserts
  • 04:12Long-Distance Migration Assay
  • 05:48Co-culture Migration and Chemo-Attraction Assays
  • 07:28Results: Migration and Chemo-Attraction Assays
  • 08:38Conclusion

Summary

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We present three simple in vitro assays-the long-distance migration assay, the co-culture migration assay, and chemo-attraction assay-that collectively evaluate the functions of human stem cell derived periventricular endothelial cells and their interaction with GABAergic interneurons.

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Keyword Extraction: MigrationChemo-attractionCo-cultureHuman Stem Cell-derived Endothelial CellsGABAergic NeuronsPeriventricular Endothelial CellsBrain DisordersAutismEpilepsySchizophreniaDepressionCell MigrationCell DissociationCell CountingTrypan Blue ExclusionNeuronal MediumEndothelial MediumCell CultureCell Assays

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