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小鼠骨骼肌肉外泄原发性肌基器的分离和分化
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Isolation and Differentiation of Primary Myoblasts from Mouse Skeletal Muscle Explants
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小鼠骨骼肌肉外泄原发性肌基器的分离和分化

DOI:
10.3791/60310-v

•

06:53 min

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October 15, 2019

•

,
1Scripps Research,Department of Molecular Medicine

Capítulos

  • 00:05Título
  • 00:46Collection and Processing of Muscle Tissue Explants
  • 02:11Harvesting Outgrowing Myoblasts
  • 04:16Expansion and Enrichment of Proliferating Myoblasts
  • 05:54Results: Myoblasts and Myotubes
  • 06:30Conclusion

Summary

Tadução automática

Tadução automática

肌细胞是增殖前体细胞，分化形成多核肌管，并最终骨骼肌肌纤维。在这里，我们提出了一个协议，从年轻的成年小鼠骨骼肌肉有效隔离和培养原发性肌体。该方法支持对培养中的肌肉细胞进行分子、遗传和代谢研究。

Tags

Keywords: Primary MyoblastsMouse Skeletal MuscleMuscle Stem CellsMyotubesCell IsolationCell CultureDifferentiationMetabolismCircadian RhythmsMuscle PhysiologyGentamycinPlating MediaMyoblast MediaTrypsinCoating Solution

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