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Capítulos
- 00:04Título
- 00:49Enzymatic Brain and Spinal Cord Digestion
- 03:58Mechanical Brain and Spinal Cord Homogenization
- 05:00Debris Removal
- 06:19Flow Cytometric Cell Staining
- 07:48Results: Representative Relative Proportions of Different Cell Types Retrieved from the CNS According to the Tissue Homogenization Method
- 09:38Conclusion
We present a flow cytometry method to identify simultaneously different cell types retrieved from mouse brain or spinal cord. This method could be exploited to isolate or characterize pure cell populations in neurodegenerative diseases or to quantify the extent of cell targeting upon in vivo administration of viral vectors or nanoparticles.
