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Prostate Organoid Cultures as Tools to Translate Genotypes and Mutational Profiles to Pharmacological Responses
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Capítulos
- 00:05Título
- 01:17Isolation, Enzymatic Digestion, and Establishments of Organoids
- 03:06Assessing Organoid Formation Capacity
- 04:05Determining Pharmacological Responses of Organoids
- 05:44Results: Organoid Seeding Efficiency and Pharmacological Response
- 07:41Conclusion
Presented here is a protocol to study pharmacological responses in prostate epithelial organoids. Organoids closely resemble in vivo biology and recapitulate patient genetics, making them attractive model systems. Prostate organoids can be established from wildtype prostates, genetically engineered mouse models, benign human tissue, and advanced prostate cancer.
