This content is Free Access.
1Department of Pharmacology and Toxicology,University of Louisville, 2Hepatobiology and Toxicology Program,University of Louisville, 3Department of Physiology and Pharmacology,West Virginia University, 4Center for Inhalation Toxicology,West Virginia University, 5Department of Pharmacology and Chemical Biology,University of Pittsburgh, 6Pittsburgh Liver Research Center,University of Pittsburgh, 7Department of Medicine, Division of Gastroenterology, Hepatology and Nutrition,University of Pittsburgh
Capítulos
- 00:04Título
- 00:42Experimental Setup and Purified Experimental Diet Acclimatization
- 01:21Vinyl Chloride (VC) Inhalation Exposure System Setup
- 02:17Pre-Exposure Setup
- 03:21Exposure Experiment and VC Concentration Validation
- 06:12Post-Exposure
- 07:01Results: Representative Effects of VC on the Enhancement of Diet-Induced Liver Injury
- 08:29Conclusion
Målet med detta protokoll var att utveckla en murin modell av låg nivå toxiskt exponering som inte orsakar öppen leverskada, utan snarare förvärar redan existerande leverskada. Detta paradigm rekapitulerar bättre mänsklig exponering och de subtila förändringar som sker vid exponering för toxiskt koncentrationer som anses säkra.
