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铁限分枝杆菌产生的细胞外囊泡的分离和特征

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Isolation and Characterization of Extracellular Vesicles Produced by Iron-limited Mycobacteria

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铁限分枝杆菌产生的细胞外囊泡的分离和特征

DOI:

10.3791/60359-v

•

08:27 min

•

October 31, 2019

•

Shamba Gupta, G. Marcela Rodriguez

¹Public Health Research Institute, New Jersey Medical School,Rutgers, The State University of New Jersey

Capítulos

00:04Título
00:47Preparation of Iron-depleted Defined Medium
01:58Growing Mycobacteria in Iron-limited Conditions
03:44Collection, Isolation and Quantification of MEVs (Mycobacterial Extracellular Vesicles)
06:38Results: MEV Purification and Quantification
07:49Conclusion

Summary

Tadução automática

结核分枝杆菌显示，由于低铁条件，细胞外囊泡的产量增加并释放。这项工作详细介绍了产生低铁条件的协议，以及针对缺铁而释放的分菌外囊泡的纯化和表征方法。

Tags

Extracellular Vesicles Mycobacteria Iron-limited Membrane Vesicles Mycobacterium Tuberculosis Mycobacterium Smegmatis Minimal Medium Metal Chelating Resin Bacterial Culture Agar Plates Spectrophotometry Late Logarithmic Phase

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