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Imunologia e Infecção

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原生聚丙烯酰胺凝胶电泳免疫溴分析内源性IRF5二聚化

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Native Polyacrylamide Gel Electrophoresis Immunoblot Analysis of Endogenous IRF5 Dimerization

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原生聚丙烯酰胺凝胶电泳免疫溴分析内源性IRF5二聚化

DOI:

10.3791/60393-v

•

08:57 min

•

October 06, 2019

•

Meijun Wang, King Hoo Lim, Kwan Ting Chow

¹Department of Biomedical Sciences,City University of Hong Kong

Capítulos

00:04Título
01:00Stimulation of CAL-1 Cells
02:23Extraction of Cellular Proteins
03:39Analysis of IRF5 Dimerization by Native PAGE
04:55Immunoblot Analysis of IRF5
06:10Results: IRF5 Dimerization
07:21Conclusion

Summary

Tadução automática

介绍了一种用于分析CAL-1血浆细胞树突状细胞系内源干扰素调节因子5二聚化的西方原生体斑点方法。该协议也可以应用于其他细胞系。

Tags

Native Polyacrylamide Gel Electrophoresis Immunoblot Analysis Endogenous IRF5 Dimerization CAL-1 Cells R848 Stimulation Cell Lysis Protein Extraction Native PAGE Western Blot

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