Detecting Establishment of Shared Blood Supply in Parabiotic Mice by Caudal Vein Glucose Injection

Xin Liu; Xue Bai; Mingqi Li; Huimin Li; Yong Zhang; Baofeng Yang

doi:10.3791/60411

Journal

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Biologia

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Detecting Establishment of Shared Blood Supply in Parabiotic Mice by Caudal Vein Glucose Injection

JoVE Journal
Biologia

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JoVE Journal Biologia

Detecting Establishment of Shared Blood Supply in Parabiotic Mice by Caudal Vein Glucose Injection

DOI:

10.3791/60411-v

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06:27 min

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February 06, 2020

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Xin Liu, Xue Bai, Mingqi Li, Huimin Li, Yong Zhang², Baofeng Yang³

¹Department of Pharmacology, The State-Province Key Laboratories of Biomedicine-Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education, College of Pharmacy,Harbin Medical University, ²Institute of Metabolic Disease,Heilongjiang Academy of Medical Science, ³Department of Pharmacology and Therapeutics, Melbourne School of Biomedical Sciences, Faculty of Medicine,Dentistry and Health Sciences University of Melbourne

Capítulos

00:04Título
01:17Parabiosis
02:33Circulation Chimerism Validation
04:13Results: Representative Changes in Blood Glucose Levels in Parabionts after Glucose Injection
05:52Conclusion

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Here we describe a new method of detecting successful establishment of shared blood circulation of two parabionts through a caudal vein injection of glucose, which causes minimal damage and is not fatal to the parabionts.

Detecting Establishment of Shared Blood Supply in Parabiotic Mice by Caudal Vein Glucose Injection

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