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测定细菌和化学物质对开牛结不二病的肠道渗透性的影响

JoVE Journal
Medicina

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JoVE Journal Medicina

Measuring the Effects of Bacteria and Chemicals on the Intestinal Permeability of Caenorhabditis elegans

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测定细菌和化学物质对开牛结不二病的肠道渗透性的影响

DOI:

10.3791/60419-v

•

07:23 min

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December 03, 2019

•

Tram Anh Ngoc Le, Baskar Selvaraj², Jae Wook Lee², Kyungsu Kang²

¹Gangneung Institute of Natural Products,Korea Institute of Science and Technology, ²Division of Bio-Medical Science & Technology,Korea University of Science and Technology (UST)

Capítulos

00:05Título
01:12Preparation of P. aeruginosa PAO1 Culture
01:58Preparation of Nematode Growth Medium (NGM) Plates for Testing the Effects of Bacteria and DIM on the Intestinal Permeability of C. elegans
02:57Bacteria or DIM Treatment and FITC-Dextran Feeding
04:19Imaging of C. elegans with the Operetta Imaging System and Determination of Intestinal Permeability
05:43Results: Effects of Bacteria on Intestinal Permeability of P. aeruginosa
06:40Conclusion

Summary

Tadução automática

该协议描述了如何测量卡内沙布迪炎的肠道渗透性。该方法有助于对肠道健康进行与肠道细菌与其宿主相互作用相关的基础生物学研究，并有助于筛选出益生菌和化学制剂，以治疗漏肠综合征和炎症性肠病。

Tags

Caenorhabditis Elegans Intestinal Permeability Bacteria Chemicals High-throughput Imaging Leaky Gut Syndrome Inflammatory Bowel Diseases P. Aeruginosa E. Coli NGM Plates DIM Plates DMSO Plates S-buffer

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