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Isolierung und Erweiterung zytotoxischer Cytokin-induzierter Killer-T-Zellen zur Krebsbehandlung
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1Department of Urology,Wan Fang Hospital, 2Department of Urology, School of Medicine, College of Medicine,Taipei Medical University, 3Graduate Institute of Clinical Medicine, College of Medicine,Taipei Medical University, 4Translational Cell Therapy Center, Department of Medical Research,China Medical University Hospital, 5Graduate Institute of Biomedical Sciences,China Medical University, 6Graduate Institute of Immunology,China Medical University, 7Department of Neurosurgery, Neuropsychiatric Center,China Medical University Hospital
Capítulos
- 00:04Introduction
- 00:50Immunophenotyping for Assessment of CIK Cells
- 02:14CD Marker Recognition
- 04:12Cytotoxic Assay
- 08:15Results: Peripheral Blood Mononuclear Cell (PBMC)-Derived CIK Expansion and Characterization and the Cytotoxicity Assay
- 10:10Conclusion
Hier stellen wir ein Protokoll zur Isolierung und Ausdehnung von peripheren mononukleären Blutzellen dar, die von Zytokin-induzierten CD3+CD56+ Killerzellen abgeleitet wurden, und ihre Zytotoxizitätswirkung gegen hämatologische und feste Krebszellen anhand eines in vitro-diagnosebasierten Durchflusszytometriesystems zu veranschaulichen.
