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Isolering och expansion av cytotoxiska Cytokin-inducerad Killer T-celler för cancerbehandling
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1Department of Urology,Wan Fang Hospital, 2Department of Urology, School of Medicine, College of Medicine,Taipei Medical University, 3Graduate Institute of Clinical Medicine, College of Medicine,Taipei Medical University, 4Translational Cell Therapy Center, Department of Medical Research,China Medical University Hospital, 5Graduate Institute of Biomedical Sciences,China Medical University, 6Graduate Institute of Immunology,China Medical University, 7Department of Neurosurgery, Neuropsychiatric Center,China Medical University Hospital
Capítulos
- 00:04Introduction
- 00:50Immunophenotyping for Assessment of CIK Cells
- 02:14CD Marker Recognition
- 04:12Cytotoxic Assay
- 08:15Results: Peripheral Blood Mononuclear Cell (PBMC)-Derived CIK Expansion and Characterization and the Cytotoxicity Assay
- 10:10Conclusion
Här presenterar vi ett protokoll för att utföra isolering och expansion av perifert blod mononukleära celler-härledda cytokin-inducerad CD3+CD56+ mördarceller och illustrera deras cytotoxicitet effekt mot hematologiska och solida cancerceller med hjälp av en in vitro diagnos flöde cytometri system.
