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使用皮肤心肌细胞进行心脏功能的体外评估
JoVE Journal
Medicina
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JoVE Journal Medicina
In Vitro Assessment of Cardiac Function Using Skinned Cardiomyocytes
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使用皮肤心肌细胞进行心脏功能的体外评估

DOI:
10.3791/60427-v

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08:19 min

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June 22, 2020

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, , , , , ,
1Unidade de Investigação Cardiovascular, Departamento de Cirurgia e Fisiologia, Faculdade de Medicina,Universidade do Porto, 2Department of Physiology,Kilimanjaro Christian Medical University College

Capítulos

  • 00:04Introduction
  • 01:00Skinned Cardiomyocyte Extraction and Permeabilization
  • 02:46Skinned Cardiomyocyte Selection and Gluing
  • 04:03Active, Passive and Calcium (Ca2+) Sensitivity Force Measurements
  • 05:58Results: Representative Skinned Cardiomyocyte Assessment
  • 07:36Conclusion

Summary

Tadução automática

Tadução automática

该协议旨在逐步描述使用皮肤心肌细胞提取和评估心脏功能的技术。这种方法允许测量和急性调节肌黄化功能使用小型冷冻活检，可以从不同的心脏位置，从小鼠到男性收集。

Tags

In Vitro Cardiac FunctionSkinned CardiomyocytesMyofilament FunctionTherapeutic InterventionsCardiomyocyte ExtractionForce TransducerTritonRelax ISO SolutionCell SuspensionRod-shaped CardiomyocyteStriation Pattern

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