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Isolation und Kultur von Okulomotor, Trochlea, und Spinal Motor Neuronen von pränatalen Islmn:GFP Transgene Mäuse
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1Department of Neurology,Boston Children’s Hospital, 2FM Kirby Neurobiology Center,Boston Children’s Hospital, 3Department of Neurology,Harvard Medical School, 4Medical Genetics Training Program,Harvard Medical School, 5Department of Ophthalmology,Boston Children’s Hospital, 6Department of Ophthalmology,Harvard Medical School, 7Broad Institute of M.I.T. and Harvard, 8Howard Hughes Medical Institute, 9Department of Neurology,Kokura Memorial Hospital, 10Department of Genetics,Albert Einstein College of Medicine
Capítulos
- 00:05Título
- 01:17Ventral Midbrain and Spinal Cord Dissection
- 06:10Tissue Dissociation
- 07:23Culture of Purified Primary Motor Neurons
- 07:59Results: Immunohistochemistry of CN3/CN4 and SMN Isolated Cultures
- 09:34Conclusion
Diese Arbeit stellt ein Protokoll vor, um homogene Zellkulturen von primären okulomotorischen, Trochlea- und Spinalmotorneuronen zu ergeben. Diese Kulturen können für vergleichende Analysen der morphologischen, zellulären, molekularen und elektrophysiologischen Eigenschaften von augen- und spinalmotorischen Neuronen verwendet werden.
