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使用CyanoGate模块化克隆工具包进行共生对蓝藻的遗传改造

JoVE Journal
Biologia

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JoVE Journal Biologia

Genetic Modification of Cyanobacteria by Conjugation Using the CyanoGate Modular Cloning Toolkit

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使用CyanoGate模块化克隆工具包进行共生对蓝藻的遗传改造

DOI:

10.3791/60451-v

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08:25 min

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October 31, 2019

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Grant A. R. Gale*^1,2,3, Alejandra A. Schiavon Osorio*^1,2, Anton Puzorjov*^1,2, Baojun Wang³, Alistair J. McCormick²

¹Institute of Molecular Plant Sciences, School of Biological Sciences,University of Edinburgh, ²Centre for Synthetic and Systems Biology,University of Edinburgh, ³Institute of Quantitative Biology, Biochemistry and Biotechnology, School of Biological Sciences,University of Edinburgh

Capítulos

00:04Título
01:11Construction of Level T Assemblies
01:43Generation of Mutants by Conjugation
06:46Results: S. elongatus UTEX 2973 and Synechocystis PCC 6803
08:03Conclusion

Summary

Tadução automática

在这里，我们提出一个协议，描述如何使用CyanoGate模块化克隆工具包组装自我复制载体，ii）通过结合将载体引入蓝藻宿主，iii）使用板读取器或流式细胞仪。

Tags

Keywords: Genetic Modification Cyanobacteria Conjugation CyanoGate Modular Cloning Synthetic Biology Renewable Biotechnology Triparental Mating Transformation Synechocystis Synechococcus E. Coli Helper Strain Plasmids

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