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Biologia do Desenvolvimento

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使用胎儿肠道有机体在体外重述吸吮到发胶过渡

JoVE Journal
Biologia do Desenvolvimento

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JoVE Journal Biologia do Desenvolvimento

Recapitulating Suckling-to-Weaning Transition In Vitro using Fetal Intestinal Organoids

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使用胎儿肠道有机体在体外重述吸吮到发胶过渡

DOI:

10.3791/60470-v

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08:15 min

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November 15, 2019

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Tânia Martins Garcia, Marit Navis, Manon E. Wildenberg, Ruurd M. van Elburg, Vanesa Muncan

¹Department of Gastroenterology and Hepatology, Tytgat Institute for Intestinal and Liver Research, Amsterdam UMC, AG&M,University of Amsterdam, ²Department of Pediatrics, Amsterdam UMC,University of Amsterdam

Capítulos

00:04Título
00:52Isolation of Fetal Small Intestinal Organoids
03:21Culturing of Fetal Organoids and Maturation Analysis at RNA and Protein Level
04:42Enzyme Activity Analysis
05:52Results: Fetal Organoid Culture
07:22Conclusion

Summary

Tadução automática

该协议描述了如何使用小鼠晚期胎儿肠道器官在体外模拟吸吮到发乳过渡，培养30天。

Tags

Fetal Intestinal Organoids Suckling-to-weaning Transition Gut Maturation Intestinal Crypts Extracellular Matrix Gel ENR Medium In Vitro Gut Model

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