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Collection of Frozen Rodent Brain Regions for Downstream Analyses
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Neurociência
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JoVE Journal Neurociência
Collection of Frozen Rodent Brain Regions for Downstream Analyses

Collection of Frozen Rodent Brain Regions for Downstream Analyses

DOI:
10.3791/60474-v

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07:06 min

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April 23, 2020

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, , ,
1Department of Psychological and Brain Sciences, Gill Center for Biomolecular Research,Indiana University

Capítulos

  • 00:04Introduction
  • 00:46Setup and Preparation
  • 03:39Dissection
  • 04:36Results: Analysis of RNA and Protein with the Frozen Brain Dissection Method
  • 06:13Conclusion

Summary

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This procedure describes the collection of discrete frozen brain regions to obtain high-quality protein and RNA using inexpensive and commonly available tools.

Tags

Frozen Brain RegionsProtein And RNA AnalysisBrain DissectionBrain MatrixLiquid NitrogenIsopentaneDry IceGlass PlateForcepsRazor BladesBrain LandmarksCD-1 Wild Type Mice

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