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1Jiangsu Key Laboratory of Neuropsychiatric Diseases and Cambridge-Suda (CAM-SU) Genomic Resource Center, Medical College of Soochow University, Department of Oncology,The First Affiliated Hospital of Soochow University, 2Department of Pathology,University of Chicago, 3Department of Pathology,Brigham and Women’s Hospital–Harvard Medical School
Capítulos
- 00:05Introduction
- 01:00Intestinal Organoid Isolation and Culture
- 04:18Flow Cytometry Analysis of EdU-Positive Cells in Enteroids
- 08:40Results: IL-22 Increases Enteroid Proliferation and Promotes Cell Death in Enteroids
- 09:46Conclusion
Le protocole présenté utilise la cytométrie de flux pour quantifier le nombre de cellules proliférantes et mortes dans les entéroïdes de souris cultivés. Cette méthode est utile pour évaluer les effets du traitement médicamenteux sur la prolifération et la survie des organoïdes.
