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角膜内皮细胞损失非侵入性激光辅助实验模型的发展

JoVE Journal
Medicina

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JoVE Journal Medicina

Development of a Noninvasive, Laser-Assisted Experimental Model of Corneal Endothelial Cell Loss

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角膜内皮细胞损失非侵入性激光辅助实验模型的发展

DOI:

10.3791/60542-v

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06:23 min

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April 24, 2020

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Annekatrin Holzhey*¹, Svenja Sonntag*², Johannes Rendenbach, Justus S. Ernesti, Vinodh Kakkassery, Salvatore Grisanti, Fred Reinholz, Sebastian Freidank, Alfred Vogel, Mahdy Ranjbar²

¹Laboratory for Angiogenesis and Ocular Cell Transplantation,University of Lübeck, ²Department of Ophthalmology,University of Lübeck, ³Institute of Biomedical Optics,University of Lübeck

Capítulos

00:04Introduction
00:44Corneal Tissue Laser Treatment and Culture
02:46Histology Preparation
03:45Hematoxylin and Eosin (H&E) Staining
05:02Results: Representative Probability of Selective Corneal Endothelial Cell (CEC) Damage
05:57Conclusion

Summary

Tadução automática

在这里，我们提出了一个协议，从Descemet的膜（DM）分离角膜内皮细胞（CEC），使用一个镉：YAG（Nd：YAG）激光作为牛角病（BK）的外活体疾病模型。

Tags

Keywords: Corneal Endothelial Cell Loss Noninvasive Model Laser-assisted Nd:YAG Laser Optical Coherence Tomography Corneal Layers Paracentesis Trephine Paraformaldehyde Cryostat Sectioning

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