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Bioparticle Microarrays for Chemotactic and Molecular Analysis of Human Neutrophil Swarming in vitro
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JoVE Journal Bioengenharia
Bioparticle Microarrays for Chemotactic and Molecular Analysis of Human Neutrophil Swarming in vitro

Bioparticle Microarrays for Chemotactic and Molecular Analysis of Human Neutrophil Swarming in vitro

DOI:
10.3791/60544-v

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11:21 min

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February 16, 2020

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1William G. Lowrie Department of Chemical and Biomolecular Engineering,The Ohio State University, 2Comprehensive Cancer Center,The Ohio State University

Capítulos

  • 00:04Introduction
  • 00:37Microfabrication of Bioparticle Microarray
  • 06:14Sample Preparation and Assay
  • 07:32Supernatant Collection
  • 08:40Results: Neutrophil Swarming Around Bioparticle Clusters
  • 10:45Conclusion

Summary

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This protocol generates bioparticle microarrays that provide spatially controlled neutrophil swarming. It provides easy access to the mediators that neutrophils release during migration and allows for quantitative imaging analysis.

Tags

Keywords: Bioparticle MicroarraysNeutrophil SwarmingIn Vitro AnalysisCytokinesLipid MediatorsMigrationMonocytesT CellsCationic PolyelectrolitePhotolithographyPolydimethylsiloxanePDMSBiopsy Punch

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