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人体神经细胞在体外热的化学和分子分析的生物粒子微阵列

JoVE Journal
Bioengenharia

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JoVE Journal Bioengenharia

Bioparticle Microarrays for Chemotactic and Molecular Analysis of Human Neutrophil Swarming in vitro

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人体神经细胞在体外热的化学和分子分析的生物粒子微阵列

DOI:

10.3791/60544-v

•

11:21 min

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February 16, 2020

•

Nicole Walters, Eduardo Reátegui²

¹William G. Lowrie Department of Chemical and Biomolecular Engineering,The Ohio State University, ²Comprehensive Cancer Center,The Ohio State University

Capítulos

00:04Introduction
00:37Microfabrication of Bioparticle Microarray
06:14Sample Preparation and Assay
07:32Supernatant Collection
08:40Results: Neutrophil Swarming Around Bioparticle Clusters
10:45Conclusion

Summary

Tadução automática

该协议生成生物粒子微阵列，提供空间控制的嗜中性粒细胞群。它便于接触嗜中性粒细胞在迁移过程中释放的介质，并允许定量成像分析。

Tags

Keywords: Bioparticle Microarrays Neutrophil Swarming In Vitro Analysis Cytokines Lipid Mediators Migration Monocytes T Cells Cationic Polyelectrolite Photolithography Polydimethylsiloxane PDMS Biopsy Punch

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