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Biologia do Desenvolvimento
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ForskydningAnalyse af myokardiemekanisk deformation (DIAMOND) afslører segmenteret heterogenitet af hjertefunktion i embryonale zebrafisk
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Displacement Analysis of Myocardial Mechanical Deformation (DIAMOND) Reveals Segmental Heterogeneity of Cardiac Function in Embryonic Zebrafish

ForskydningAnalyse af myokardiemekanisk deformation (DIAMOND) afslører segmenteret heterogenitet af hjertefunktion i embryonale zebrafisk

DOI:
10.3791/60547-v

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09:15 min

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February 06, 2020

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,
1Division of Cardiology, Department of Medicine,David Geffen School of Medicine at University of California, Los Angeles, 2Ronald Reagan UCLA Medical Center, 3Veterans Affairs West Los Angeles Medical Center

Capítulos

  • 00:04Introduction
  • 01:13Reconstruction of the 3D Systolic and Diastolic Heart and Segmentation of the Ventricle
  • 02:41Creation of Rectangular Parallelepipeds for Image Registration and Resampling of Systolic and Diastolic 3D Hearts Along the Short Axis Plane
  • 05:01Division of the Resampled Heart
  • 06:20Registration of Systolic and Diastolic Image Matrices and Output of Displacement Vectors
  • 07:01Results: Segmental Heterogeneity in Cardiac Function and Susceptibility to Chemotherapy-induced Injury
  • 08:45Conclusion

Summary

Tadução automática

Tadução automática

Formålet med denne protokol er at detaljere en ny metode til vurdering af segmental hjertefunktion hos embryonale zebrafisk under både fysiologiske og patologiske tilstande.

Tags

– Displacement Analysis Of Myocardial Mechanical Deformation (DIAMOND)- Segmental Cardiac Function- Zebrafish Embryos- Quantitative Assessment- Four-dimensional- Cardiac Mechanics- Doxorubicin-induced Cardiac Toxicity- High-throughput In Vivo Screening- Mammalian Heart- Muscle Fiber Orientation- Rotational And Twisting Motion- Anatomical Structure- Ventricle Segmentation- 3D TIF Files- Image Analysis Software- Prepimage 1.m

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