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Biologia do Desenvolvimento
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Cell-cell Fusion of Genome Edited Cell Lines for Perturbation of Cellular Structure and Function
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Cell-cell Fusion of Genome Edited Cell Lines for Perturbation of Cellular Structure and Function

Cell-cell Fusion of Genome Edited Cell Lines for Perturbation of Cellular Structure and Function

DOI:
10.3791/60550-v

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07:30 min

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December 07, 2019

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,
1Photonics Group, Department of Physics,Imperial College London, 2The Medical Research Council Cancer Unit, Hutchison/MRC Research Centre, Cambridge Biomedical Campus,University of Cambridge, 3Cambridge Institute for Medical Research, Cambridge Biomedical Campus,University of Cambridge

Capítulos

  • 00:04Título
  • 00:29Differential Fluorescent Cell Labeling
  • 02:00Cell-Cell Fusion
  • 03:03Fused Cell Enrichment
  • 04:18Cell-Cell Fusion Immunofluorescent Staining and Imaging
  • 05:30Results: Representative Fused Cell Flow Cytometry Enrichment and Imaging
  • 06:47Conclusion

Summary

Tadução automática

Tadução automática

The purpose of this protocol is to fuse two different cell types to create hybrid cells. Fluorescence microscopy analysis of fused cells is used to track the cell of origin of cellular organelles. This assay can be used to explore how cellular structure and function respond to perturbation by cell fusion.

Tags

Cell-cell FusionGenome EditingCellular StructureCellular FunctionFluorescent LabelingDye LabelingCell CultureCell FusionPolyethylene Glycol (PEG)Cell ImagingOrganelle HomeostasisOrganelle Function

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