Journal
/
Genética
/
Use of the Pyrimidine Analog, 5-Iodo-2′-Deoxyuridine (IdU) with Cell Cycle Markers to Establish Cell Cycle Phases in a Mass Cytometry Platform
JoVE Journal
Genética
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Genética
Use of the Pyrimidine Analog, 5-Iodo-2′-Deoxyuridine (IdU) with Cell Cycle Markers to Establish Cell Cycle Phases in a Mass Cytometry Platform

Use of the Pyrimidine Analog, 5-Iodo-2′-Deoxyuridine (IdU) with Cell Cycle Markers to Establish Cell Cycle Phases in a Mass Cytometry Platform

DOI:
10.3791/60556-v

•

08:37 min

•

October 22, 2021

•

,
1Ohio State Comprehensive Cancer Center, Division of Hematology,The Ohio State University

Capítulos

  • 00:04Introduction
  • 00:515‐Iodo‐2’‐Deoxyuridine (IdU) Incorporation and Sample Preservation
  • 02:28Data Analysis
  • 06:27Results: Representative Cell Cycle Phase Establishment
  • 07:55Conclusion

Summary

Tadução automática

Tadução automática

This protocol adapts cell cycle measurements for use in a mass cytometry platform. With the multi-parameter capabilities of mass cytometry, direct measurement of iodine incorporation allows identification of cells in S-phase while intracellular cycling markers enable characterization of each cell cycle state in a range of experimental conditions.

Tags

Keywords: Cell CycleMass Cytometry5-Iodo-2′-Deoxyuridine (IdU)Cell Cycle MarkersAcute Myeloid LeukemiaStem And Progenitor CellsCell GrowthCell ProliferationLive/dead StainParaformaldehydeCell Staining MediumDMSOIridium

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/60556?article_type=v

High-Throughput Robotically Assisted Isolation of Temperature-sensitive Lethal Mutants in Chlamydomonas reinhardtii
check_url/pt/60556?article_type=v

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus
check_url/pt/60556?article_type=v

Detection of Copy Number Alterations Using Single Cell Sequencing
check_url/pt/60556?article_type=v

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format
check_url/pt/60556?article_type=v

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
check_url/pt/60556?article_type=v

Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System
check_url/pt/60556?article_type=v

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry
check_url/pt/60556?article_type=v

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
check_url/pt/60556?article_type=v

Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes
check_url/pt/60556?article_type=v

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models

Read Article