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Anvendelse af membran og cellevæg selektive fluorescerende farvestoffer til levende celle billeder af filamentøse svampe
JoVE Journal
Biologia
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JoVE Journal Biologia
Application of Membrane and Cell Wall Selective Fluorescent Dyes for Live-Cell Imaging of Filamentous Fungi

Anvendelse af membran og cellevæg selektive fluorescerende farvestoffer til levende celle billeder af filamentøse svampe

DOI:
10.3791/60613-v

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07:44 min

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November 28, 2019

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,
1Department of Microbiology,University of Innsbruck

Capítulos

  • 00:04Título
  • 01:06Preparation of Fungal Samples
  • 01:40Sample Preparation for Microscopy
  • 02:57Live-cell Microscopy
  • 04:16Results: Live-Cell Imaging of Filamentous Fungi
  • 06:47Conclusion

Summary

Tadução automática

Tadução automática

Vitale fluorescerende farvestoffer er essentielle værktøjer til analyser af levende celler i moderne svampe cellebiologi. Dette papir beskriver anvendelsen af etablerede og mindre kendte fluorescerende farvestoffer til sporing af plasma membran dynamik, endo-/exocytose og cellevæg morfogenesis i filamentøse svampe.

Tags

Keywords: Membrane DyesCell Wall DyesLive-cell ImagingFilamentous FungiFungal CellsOrganelle DynamicsVital StainingImage-guided DiagnosisAspergillosisDrug Uptake DynamicsSample PreparationMicroscopyImage Acquisition Settings

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